![]() ![]() ![]() Information from each of these images is merged computationally to reconstruct a three-dimensional volume. A series of transmission electron microscopy images of the specimen are obtained while tilting the specimen to various angles. This process preserves the native state of the specimen through the imaging process. A biological specimen (such as a protein, nucleic acid, macromolecular complex, virion, organelle, or cell) is vitrified in a thin film of aqueous solution. ![]() Cryo-electron tomography is an experimental technique to obtain a three-dimensional representation of a sample preserved in amorphous ice.
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